The study on recognition of polydiacetylene functionalized with mannopyranoside mimic membrance for Escherichia coli

People has known that the recognition between cells and bacteria is related to the glycolipid, as a receptor on the cell surface. Diacetylene moiety i n the hydrocarbon region can be cross-linked by UV irradiation to form the corresponding polydiacetylene polymer. Color changes of polydiacetylenes ha ve been known to occur in response to a variety of environmental perturbati ons such as heating, pH, mechanical stress or in the case of biosensor, due to the strong pi-pi* absorption of the conjugation backbone. In this repor t, polydiacetylene mixed with mannopyranoside monolayer is prepared by Lang muir-Blodgett film technology, E. coli K-12 adheres on the monolayer surfac e after gram strain. The result shows that the mannopyraoside could be reco gnitized by E. coli, but couldn't be recognitized by O111B4 and S, flexneri .