The solution structure of cytochrome b(5) mutant (E44A/E48A/E56A/D60A)

Authors
Wu, YB Lu, J Qian, CM Li, EC Wang, JF Tang, WX Zhu, DX Wang, YH Wang, WH Lu, JX Xie, Y Huang, ZX
Citation
Yb. Wu et al., The solution structure of cytochrome b(5) mutant (E44A/E48A/E56A/D60A), CHEM J CH U, 21(11), 2000, pp. 1631-1632
Citations number
9
Categorie Soggetti
Chemistry
Journal title
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
ISSN journal
02510790 → ACNP
Volume
21
Issue
11
Year of publication
2000
Pages
1631 - 1632
Database
ISI
SICI code
0251-0790(200011)21:11<1631:TSSOCB>2.0.ZU;2-H
Abstract
The solution structure of bovine cytochrome b(5) mutant (E44A/E48A/E56A/D60 A) has been obtained from H-1 NMR spectra measured at 600 MHz. The final RM SD values with respect to the average structure are (0. 056 +/- 0. 009) nm and (0. 105 +/- 0. 010) nm for backbone and all heavy atoms, respectively. The solution structure, when compared with the X-ray structure of wild type , has no significant changes in the whole folding and secondary structure. Small changes are restricted to local conformation near mutated sites.