To investigate the interaction of hTCF4, the yeast two-hybrid system has be
en used for testing the interaction of mutants of hTCF4 with themselves. Mu
tants of hTCF4(hTCF4 I) and hTCF4 II) have been obtained by polymerase chai
n reaction (PCR). Bait (hTCF4 I-pDBLeu and hTCF4 II-pDBLeu) and prey (hTCF4
I-pPC86 and hTCF4 II-pPC86) have been constructed by DNA recombination for
yeast two-hybrid. Interaction of hTCF4 II with itself is found in the reve
rse yeast two-hybrid system (GIBCOBRL Co.). However, no interactions are fo
und in hTCF4 II with hTCF4 I and in hTCF4 I with hTCF4 I. These results sug
gest that hTCF4 could interact with itself to form homodimer or homocopolym
er and perform the transcriptional activating function through LZ or HLH mo
tifs in nucleus of renal cell carcinoma.