NONADRENERGIC NONCHOLINERGIC REGULATION O F STONE-DISEASED AND STONE-FREE HUMAN GALLBLADDERS

Citation
P. Sanger et al., NONADRENERGIC NONCHOLINERGIC REGULATION O F STONE-DISEASED AND STONE-FREE HUMAN GALLBLADDERS, Zentralblatt fur Chirurgie, 122(5), 1997, pp. 418-424
Citations number
36
Categorie Soggetti
Surgery
Journal title
ISSN journal
0044409X
Volume
122
Issue
5
Year of publication
1997
Pages
418 - 424
Database
ISI
SICI code
0044-409X(1997)122:5<418:NNROFS>2.0.ZU;2-I
Abstract
This study is to determine the role of nitric oxide (NO), as primary n eurotransmitter of the non-adrenergic noncholinergic (NANC) innervatio n, of stone-diseased and stone-free human gallbladders. Human gallblad der muscle strips were mounted in modified Krebs-Henseleit-solution wi th atropine (1 mu M), guanethidine sulf. (5 mu M) and aerated with Car bogen. Electrical field stimulation (EFS, 70V, 0.5 ms, 100 pulses) was used at frequencies of 1, 3, 10 Hz to activate NANC nerves. L-omega-n itro-L-arginine (L-NNA, 100 mu M), L-arginine (L-ARG, 120 mu M) was us ed to manipulate the NO-synthase. Gallbladder slices of 3 mu m were st ained by means of APAAP-method (alkaline phosphatase anti alkaline pho sphatase) for histological examination. In the control group (basal to ne = 8.94+/-1.17 mN) EFS caused a frequency dependent reduction of bas al tone (1 Hz = 5.73 +/- 0.81 mN; 3 Hz = 5.18 +/- 0.65 mN; 10 Hz = 4.6 3+/-0.49 mN). Incubation with L-NNA increased the tone (7.63+/-0.76 mN ), Contractor group (basal tone 7.79+/-0.93 mN) reacted like the contr ol group but frequency independent and additionally with spontaneous p hasic contractions. In the non-contractor group (basal tone 4.13+/-0.6 5 mN) EFS only decreased the frequency of spontaneous phasic contracti ons. L-NNA caused an increase in tone (5.97 +/- 0.84 mN) and frequency ; L-Arginine significantly reversed this effect. Histology: Contractor group showed wrinkled mucosal membrane and mild grade of inflammation . Shallow mucosa, necrosis and high grade of inflammation were found i n the non-contractor group. Conclusions: 1. In vitro, NANC-relaxation of human gallbladder is NO dependent. 2. Motility of stone-diseased ga llbladders is modulated by NO and seems to depend on the degree of sca rrification.