Increased production of IL-4 by gut T-cell lines from patients with dermatitis herpetiformis compared to patients with isolated gluten-sensitive enteropathy
Rp. Hall et al., Increased production of IL-4 by gut T-cell lines from patients with dermatitis herpetiformis compared to patients with isolated gluten-sensitive enteropathy, DIG DIS SCI, 45(10), 2000, pp. 2036-2043
Dermatitis herpetiformis (DH) and isolated gluten-sensitive enteropathy (GS
E) are gluten-sensitive diseases in which ingestion of dietary gluten resul
ts in the development of clinical disease. Patients with DH develop cutaneo
us IgA deposits and a severe skin disease, but rarely develop gastrointesti
nal symptoms. Patients with isolated GSE develop clinically significant gas
trointestinal symptoms, but not skin disease or cutaneous IgA deposits. The
aim of this study was to investigate the mechanism by which a mucosal immu
ne response to the same dietary antigen can result in two distinct clinical
phenotypes. T-cell lines were derived from activated T-cells in the small
bowel mucosa of five patients with DH and 14 patients with isolated GSE and
analyzed for T-cell markers and cytokine production in vitro. T-cell lines
from DH and isolated GSE patients produced IFN-gamma after stimulation (me
an: DPI = 2619 pg/ml; isolated GSE = 1993 pg/ml; NS). T-cell lines from pat
ients with DH, however, produced significantly more IL-4 than the T-cell li
nes from patients with isolated GSE (IL-4: DH = 2010 pg/ml; isolated GSE =
235 pg/ml; P < 0.05). Analysis of intracytoplasmic cytokine production by t
he T-cell lines showed that T-cell lines from patients with DH were CD4(+)
predominant, with a greater proportion of CD4(+)/IL4(+) cells than CD4(+)/I
FN-<gamma>(+) cells. In contrast, isolated GSE T-cell lines were predominan
tly CD8(+), with an equal proportion of IL-4; and IFN-gamma -positive cells
. These studies demonstrate that T cell lines from patients with DH produce
significantly more IL-4 than T-cell lines from patients with isolated GSE,
while producing similar amounts of IFN-gamma. This difference in cytokine
pattern may play an important role in the different clinical manifestations
of these two forms of gluten sensitivity.