Electrochemical flow immunoassay using capillary column and ferrocene conjugated immunoglobulin G

This paper describes a miniaturized now immunoassay system. Ferrocene (Fc) conjugated with anti-HCG immunoglobulin G (IgG) antibody (Fc-IgG) was prepa red and used as a novel analytical reagent. The system consists of the inun unoreaction part, the capillary column packed with cation exchange resin, a nd the flow cell for electrochemical detection of Fc-lgG. The assay yielded a linear relationship between signal and HCG concentration in the range of 0-2000 mlU/mL (L = dm(3)). This electrochemical now immunoassay requires m inute quantities of serum and generates highly reproducible results.