We developed a novel method for the fabrication of a multichannel glutamate
sensor based on a planar electrode array. By scanning and holding the elec
trode potential in a solution containing glutamate oxidase and a polymer to
which horseradish peroxidase and electron-transfer mediator were bound, we
successfully immobilized these enzymes and a mediator on the electrodes of
the electrode array through electrochemical deposition of the polymer. We
confirmed the resulting sensor possessed high sensitivity and selectivity t
o glutamate. This multichannel sensor may be useful for the non-invasive, r
eal-time monitoring of the glutamate distribution in biological samples.