Electrofusion prompts regeneration of yeast protoplasts: Effect of electric pulse and media on the regeneraion efficiency

Regeneration efficiency of protoplasts is one of very important factors in breeding of yeasts using electrofusion. When both an alternative current (0 .4 kV/cm, 2 MHz) for 10 s and 5 times of directive pulses (5.0 kV/cm, 50 mu s) were succesively charged to yeast protoplasts in the medium containing s orbitol (0.8 M) and MgCl2(0.2 mM), about 60 folds increase in the regenerat ion efficiency was observed with increase of average diameters of the proto plasts compared to the efficiency before electrofusion. Electrofusian was f ound to increase the regeneration efficiency of the yeast protoplasts.