Measurement of Helicobacter pylori using anti its urease monoclonal antibody by surface plasmon resonance

Adults over 70% of Japanese are infected by Helicobacter pylori causing chr onic gastrisis and gastric ulcer. In this study by employing a unique monoc lonal antibody against Helicobacter pylori urease, the bacterium was detect ed down to 2 x 10(7) cell/ml using a SPR apparatus equipping two detecting cells. Ultrasonicated sample of Heticobacter pylori gave a huge improvement in the detection limit rather than the non-ultrasonicated sample by 100 fo ld. Escherichia coli was hardly detected, suggesting the specific detection of Helicobacter Pylori by this measurement system.. Using this type of SPR apparatus, the wider concentration of Helicobacter pylori could be detecte d comparing with the conventional assay kit, while the detection limit of t he SPR system was worse by 10 fold.