In order to reduce the content of nitrogen in treated wastewater, microbial
processes conducting nitrification of nitrogenous compounds and denitrific
ation to gaseous nitrogen are included in the sewage wastewater treatment p
rocess. A set of compounds has been found inhibitory to these microorganism
s. A procedure is presented for identification of nitrification inhibitors
in wastewaters. This includes fractionation of the wastewater sample and lo
cation of the inhibitory effect using a nitrification inhibition assay wher
e pure cultures of Nitrobacter are used. A series of unsaturated fatty acid
s and two monoterpenes were found to constitute the inhibitory effect in a
wastewater sample from a plant for drying wood-derived fuel. (C) 2000 by Jo
hn Wiley & Sons, Inc.