A. Medvedovici et al., Liquid extraction and HPLC-DAD assay of hydrochlorothiazide from plasma for a bioequivalence study at the lowest therapeutic dose, EUR J DRUG, 25(2), 2000, pp. 91-96
Citations number
22
Categorie Soggetti
Pharmacology & Toxicology
Journal title
EUROPEAN JOURNAL OF DRUG METABOLISM AND PHARMACOKINETICS
The main parameters considered in optimizing the liquid extraction and quan
titative assay were the yield, precision, limit of quantification, time req
uired for extraction and concentration, and quantity of solvent. The influe
nce on these parameters of the following factors was examined: nature of th
e extracting solvent, quantity of solvent, co-extraction solvent, and durat
ion of stirring. instead of equilibrium parameters of the involved thermody
namic system, a kinetic approach was preferred in terms of the effective pa
rtition 'constant', which is not really constant but a function of time and
extraction conditions. The final selected method, considered to be rapid a
nd simple, was applied to determine the pharmacokinetics of hydrochlorotiaz
ide (HCT) after administration of Capozide (Bristol-Myers Squibb) tablets c
ontaining 50 mg Captopril and 25 mg HCT, to 4 healthy volunteers. The resul
ts obtained were in accordance with the pharmacokinetic parameters of HCT r
eported in the literature.