Fe. Loffler et al., COMPLETE REDUCTIVE DECHLORINATION OF 1,2-DICHLOROPROPANE BY ANAEROBIC-BACTERIA, Applied and environmental microbiology, 63(7), 1997, pp. 2870-2875
The transformation of 1,2-dichloropropane (1,2-D) was observed in anae
robic microcosms and enrichment cultures derived from Red Cedar Creek
sediment. 1-Chloropropane (1-CP) and 2-CP were detected after an incub
ation period of 4 weeks. After 1 months the initial amount of 1,2-D wa
s stoichiometrically converted to propene, which was not further trans
formed. Dechlorination of 1,2-D was not inhibited by 2-bromoethanesulf
onate. Sequential 5% (vol/vol) transfers from active microcosms yielde
d a sediment-free, nonmethanogenic culture, which completely dechlorin
ated 1,2-D to propene at a rate of 5 nmol min(-1) mg of protein(-1). N
o intermediate formation of 1-CP or 2-CP was detected in the sediment-
free enrichment culture. A variety of electron donors, including hydro
gen, supported reductive dechlorination of 1,2-D. The highest dechlori
nation rates were observed between 20 degrees and 25 degrees C. In the
presence of 1,2-D, the hydrogen threshold concentration was below 1 p
pm by volume (ppmv). In addition to 1,2-D, the enrichment culture tran
sformed 1,1-D,2-bromo-1-CP, tetrachloroethene, 1,1,2,2-tetrachloroetha
ne, and 1,2-dichloroethane to less halogenated compounds. These findin
gs extend our knowledge of the reductive dechlorination process and sh
ow that halogenated propanes can be completely dechlorinated by anaero
bic bacteria.