Blockade of TGF-beta by in vivo gene transfer of a soluble TGF-beta type II receptor in the muscle inhibits corneal opacification, edema and angiogenesis

Accumulating evidence suggests the involvement of TGF-beta in the process o f corneal opacity, which is one of the serious causes of visual loss. Howev er, whether TGF-beta is indeed critical for the pathogenesis remains unknow n. We constructed an adenovirus expressing an entire ectodomain of the huma n type II TGF-beta receptor fused to Fc portion of human IgG (AdT beta -ExR ): this soluble receptor is secreted from AdT beta -ExR-infected cells, bin ds to TGF-beta and inhibits TGF-beta signaling. When AdT beta -ExR was inje cted into the femoral muscle of Balb/c mice, a high level of the soluble re ceptor protein (2.0-3.5 x 10(3) pM) was detectable in the serum and in the ocular fluid for at least 10 days. In the mice subjected to corneal injury with silver nitrate and to intramuscular injection with either saline or a control adenovirus expressing beta -galactosidase (AdLacZ), corneal opacifi cation composed of extracellular matrix (ECM) accumulation, of infiltration of neutrophils and monocytes/macrophages, and of angiogenesis were all ind uced. In contrast, they were markedly reduced in the mice injected with AdT beta -ExR. Immunohistochemical analysis revealed that TGF-beta, fibronecti n, macrophage chemoattractant protein-1, and vascular endothelial growth fa ctor were densely stained in the edge of wounded cornea, but they were scar cely present in the injured-cornea of AdT beta -ExR-treated mice. Our resul ts demonstrate that TGF-beta indeed plays a critical role in the process of cornea opacification, and that adenovirus-mediated expression of a soluble TGF-beta receptor can be therapeutically useful.