Restriction profile studies on local isolates of IBD virus

Authors
Parthiban, M Thiagarajan, V Ramadass, P
Citation
M. Parthiban et al., Restriction profile studies on local isolates of IBD virus, I J ANIM SC, 70(11), 2000, pp. 1105-1107
Citations number
13
Categorie Soggetti
Animal Sciences
Journal title
INDIAN JOURNAL OF ANIMAL SCIENCES
ISSN journal
03678318 → ACNP
Volume
70
Issue
11
Year of publication
2000
Pages
1105 - 1107
Database
ISI
SICI code
0367-8318(200011)70:11<1105:RPSOLI>2.0.ZU;2-5
Abstract
Five local isolates of infectious bursal disease virus from different geogr aphical area of Tamil Nadu were characterized by RT-PCR-RE assay to find ou t the genomic variants of IBDV. The variable region of VP2 of IBDV was ampl ified by RT-PCR techinque using IBDV specific primers. The amplified produc t was subjected to different restriction enzymes digestion, viz. Hac III, S au 3 AI and Alu I. The enzyme Alu I was useful to differentiate the local i solates at genomic level.