GRADIENT-ENHANCED ONE-DIMENSIONAL PROTON CHEMICAL-SHIFT CORRELATION WITH FULL SENSITIVITY

High-quality spectra were obtained by implementing pulsed field gradie nts (PFGs) as part of 1D selective experiments. The use of PFGs for co herence rejection rather than coherence selection ensures that there i s no loss of signal and the sensitivity of these experiments is the sa me as that of their phase-cycled predecessors. The excitation scheme c hosen ensures that these experiments are highly resistant to spin-spin relaxation. The following techniques are described: 1D ge-TOCSY, 1D g e-NOESY, 1D ge-TOCSY-TOCSY, 1D ge-NOESY-NOESY, 1D ge-TOCSY-NOESY, and 1D ge-NOESY-TOCSY. Their applications, for the separation of overlappi ng spin systems, tracing spin-diffusion signals, and extending the tra nsfer of magnetization beyond an individual spin system, are illustrat ed using oligo- and polysaccharide samples. (C) 1997 Academic Press.