Integron-located VEB-1 extended-spectrum beta-lactamase gene in a Proteus mirabilis clinical isolate from Vietnam

A clinical isolate of Proteus mirabilis Lil-1 was obtained from a Vietnames e patient hospitalized in Paris, France. This isolate was resistant to ceph alosporins, and there was marked synergy between cephalosporins and clavula nic acid together with unusual synergy between cefoxitin and cefuroxime. PC R analysis revealed the presence of bla(VEB-1), an integron-located gene co ding for an extended-spectrum beta -lactamase (ESBL) identified previously in an Escherichia coli isolate MG-1 from Vietnam. Using class 1 integron pr imers and bla(VEB-1) intragenic primers, the insert region of the bla(VEB-1 )-containing integron along with flanking sequences were amplified from P.m irabilis Lil-1 whole-cell DNA. A novel class 1 integron, ln55, was identifi ed that contained, in addition to intl1, qacE Delta1, sul1 and Orf5 genes, an 8 kb variable region. This region was comparable in size to that found p reviously in E. coli MG-I, but different from those previously identified i n two Pseudomonas aeruginosa isolates from Thailand. ln55 was located on a 190 kb self-transferable plasmid, which was different in size and structure from that found in E. coli MG-1. The finding of bla(VEB-1) on different pl asmids and integrons in enterobacterial isolates underlines the interspecie s spread of this novel ESBL gene.