MOLECULAR-CLONING AND SEQUENCING OF THE CDNA-ENCODING THE CATALYTIC SUBUNIT OF MOUSE GLUTAMATE-CYSTEINE LIGASE

Authors
REID LL BOTTA D LU Y GALLAGHER EP KAVANAGH TJ
Citation
Ll. Reid et al., MOLECULAR-CLONING AND SEQUENCING OF THE CDNA-ENCODING THE CATALYTIC SUBUNIT OF MOUSE GLUTAMATE-CYSTEINE LIGASE, Biochimica et biophysica acta, N. Gene structure and expression, 1352(3), 1997, pp. 233-237
Citations number
10
Categorie Soggetti
Biology,Biophysics,"Biothechnology & Applied Migrobiology
Journal title
Biochimica et biophysica acta, N. Gene structure and expression → ACNP
ISSN journal
01674781
Volume
1352
Issue
3
Year of publication
1997
Pages
233 - 237
Database
ISI
SICI code
0167-4781(1997)1352:3<233:MASOTC>2.0.ZU;2-9
Abstract
Reverse transcription-polymerase chain reaction (RT-PCR) was used for the enzymatic synthesis of cDNA sequences encompassing the open readin g frame for the catalytic subunit of mouse kidney glutamate-cysteine l igase (Glclc). Comparison of the mouse Glcle cDNA sequence and predict ed protein sequence with that of rat Glcle and human GLCLC revealed be tween 94.8% and 88.4% cDNA homology and 98.4% to 95% amino acid identi ty, respectively. Published by Elsevier Science B.V.