SEQUENCE POLYMORPHISM AND STRUCTURAL-ANALYSIS OF TIMOTHY GRASS-POLLENPROFILIN ALLERGEN (PHL-P-11)

Authors
ASTURIAS JA ARILLA MC BARTOLOME B MARTINEZ J MARTINEZ A PALACIOS R
Citation
Ja. Asturias et al., SEQUENCE POLYMORPHISM AND STRUCTURAL-ANALYSIS OF TIMOTHY GRASS-POLLENPROFILIN ALLERGEN (PHL-P-11), Biochimica et biophysica acta, N. Gene structure and expression, 1352(3), 1997, pp. 253-257
Citations number
25
Categorie Soggetti
Biology,Biophysics,"Biothechnology & Applied Migrobiology
Journal title
Biochimica et biophysica acta, N. Gene structure and expression → ACNP
ISSN journal
01674781
Volume
1352
Issue
3
Year of publication
1997
Pages
253 - 257
Database
ISI
SICI code
0167-4781(1997)1352:3<253:SPASOT>2.0.ZU;2-N
Abstract
Three cDNA clones encoding timothy grass pollen profilin (Phl p 11) we re newly isolated. Comparison of the sequences of four cDNA clones, in cluding a previously isolated clone, showed a low level of polymorphis m. Isoelectrofocusing of highly purified timothy grass profilin indica ted the existence of at least five isoforms. One recombinant profilin showed similar immunological properties to natural timothy grass profi lin. Tertiary structure of Phleum pratense profilin was obtained by ho mology-based molecular modeling. (C) 1997 Elsevier Science B.V.