Cl. Armstrong et al., Expression of heat-shock protein Hsp25 in mouse Purkinje cells during development reveals novel features of cerebellar compartmentation, J COMP NEUR, 429(1), 2001, pp. 7-21
The small heat shock protein Hsp25 is constitutively expressed in the adult
mouse cerebellum by parasagittal stripes of Purkinje cells confined to the
caudal central zone (similar to lobules VI and VII), the nodular zone (sim
ilar to ventral lobule IX and lobule X), and the paraflocculi/flocculi. Dur
ing development several distinct phases in Hsp25 expression can be distingu
ished. Hsp25-immunopositive Purkinje cells are first seen at birth, when fo
ur clusters are visible in the vermis of lobules NN, and scattered Hsp25-im
munoreactive Purkinje cells are seen in lobule VIII. By postnatal day 2/3,
six narrow parasagittal stripes of Hsp25-immunopositive Purkinje cells are
seen in the vermis of the anterior lobe. In the posterior lobules, most Pur
kinje cells in the vermis of lobules VIII and IX express Hsp25. This initia
l limited expression is followed by a phase of widespread expression (postn
atal days 6-9) in which Hsp25 immunoreactivity is detected in virtually all
Purkinje cells. This global cerebellar expression of Hsp25 then gradually
disappears, first in the anterior zone and the hemispheres and subsequently
in the posterior zone, to leave the restricted adult expression pattern. W
estern blotting analysis and immunoprecipitation with anti-Hsp25 suggest th
at all immunocytochemistry can be attributed the expression of Hsp25. Furth
ermore, visual deprivation had no effect on the development of Hsp25 expres
sion in Purkinje cells, suggesting that visuomotor input is not responsible
for the establishment of constitutive Hsp25 expression in the cerebellar c
ortex. (C) 2001 'Wiley-Liss, Inc.