PLACENTA GROWTH-FACTOR - IDENTIFICATION AND CHARACTERIZATION OF A NOVEL ISOFORM GENERATED BY RNA ALTERNATIVE SPLICING

Citation
Yh. Cao et al., PLACENTA GROWTH-FACTOR - IDENTIFICATION AND CHARACTERIZATION OF A NOVEL ISOFORM GENERATED BY RNA ALTERNATIVE SPLICING, Biochemical and biophysical research communications, 235(3), 1997, pp. 493-498
Citations number
31
Categorie Soggetti
Biology,Biophysics
ISSN journal
0006291X
Volume
235
Issue
3
Year of publication
1997
Pages
493 - 498
Database
ISI
SICI code
0006-291X(1997)235:3<493:PG-IAC>2.0.ZU;2-V
Abstract
We report the isolation and characterization of a third isoform of pla centa growth factor (PlGF), generated by alternative splicing of its R NA transcript. This novel form of PlGF, PlGF-3, was cloned by the poly merase chain reaction technique using a human cDNA library prepared fr om the terminal placental tissue. PlGF-3 contains an in-frame insertio n of 72-amino acids near the C-terminal portion of PlGF-1. Southern bl ot analysis revealed that a single gene encoded PlGF-2 and PlGF-3. Nuc leic acid sequence analysis found the insertion of 216 nucleotides of PlGF-3 between exon 4 and exon 5 of the PlGF gene. Northern blot and t issue distribution studies discovered two mRNA species for PlGF-3, whi ch were both uniquely expressed in the placenta. Transient expression of PlGF-3 cDNA in mammalian cells showed PlGF-3 was detected in the co nditioned medium as both dimers and monomers. Unlike PlGF-2, PlGF-3 la cked heparin-binding affinity. Thus, alternative splicing of PlGF RNA produces at least three polypeptides with different secretion pattern, heparin-binding affinity and dimerization properties. (C) 1997 Academ ic Press.