Enzymatic synthesis of disaccharides by beta-galactosidase-catalyzed glycosylation of a glycocluster

Authors
de Santiago, R Fernandez-Mayoralas, A Garcia-Junceda, E
Citation
R. De Santiago et al., Enzymatic synthesis of disaccharides by beta-galactosidase-catalyzed glycosylation of a glycocluster, J MOL CAT B, 11(2-3), 2000, pp. 71-79
Citations number
12
Categorie Soggetti
Physical Chemistry/Chemical Physics
Journal title
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
ISSN journal
13811177 → ACNP
Volume
11
Issue
2-3
Year of publication
2000
Pages
71 - 79
Database
ISI
SICI code
1381-1177(200012)11:2-3<71:ESODBB>2.0.ZU;2-3
Abstract
The synthesis of disaccharides by galactosylation of a glucose-containing c luster has been studied using beta -galactosidases from different sources. Galactosylation of the cluster could be detected by ESI-MS. After size-excl usion chromatography of the reaction mixtures and subsequent cleavage of th e linker, galactopyranosyl-glucose disaccharides were obtained with yield a nd regioselectivity dependent on the origin of the enzyme. The possibility of using this method for the synthesis of multivalent glycoconjugates is di scussed. (C) 2000 Elsevier Science B.V. All rights reserved.