Automatic analysis for amperometrical recordings of exocytosis

Amperometry is a widely used technique for monitoring the secretion of cate cholamines (CA) by exocytosis. The use of carbon fibre microelectrodes allo ws the on-line recording of CA released from a single secretory vesicle. Am perometric signals are generated by oxidation of the quantally released CA close to the electrode tip. Each event of exocytosis is called a secretory spike. Here we describe a program written for IGOR (Wavemetrics, Lake Osweg o, OR, USA), which may be used to analyze amperometric signals off-line. Th e procedures allow, (i) digital filtering and analysis of the current noise , spike identification and calculation of spike kinetic parameters: (ii) sp ike review; (iii) pooling spikes and data to create galleries, tables and h istograms of measured parameters which can be exported to a graphic format or files for further analysis. (C) 2000 Elsevier Science B.V. All rights re served.