H. Lechat et al., Structure and distribution of glucomannan and sulfated glucan in the cell walls of the red alga Kappaphycus alvarezii (gigartinales, rhodophyta), J PHYCOLOGY, 36(5), 2000, pp. 891-902
Two new polysaccharides were isolated from the cell walls of the carrageena
n producing red seaweed Kappaphycus alvarezii (Doty) Doty, They were charac
terized by chemical analyses, enzymatic degradations, and nuclear magnetic
resonance spectroscopy, One was a 4.0 M NaOH soluble beta-(1,4)-D-glucomann
an that mostly precipitated upon neutralization and dialysis, It was compos
ed of about 82 residues, and 70% of its glucose and mannose were released b
y a commercial cellulase enzyme complex, The disaccharide beta -D-Man (1-->
4) D-Glc was recovered from the hydrolysate during the first hours of degra
dation and confirmed the chemical structure of the polysaccharide, The othe
r polysaccharide was extracted with 1.5 M NaOH and was identified as a sulf
ated glucan of degree of polymerization of about 180 1,4-linked beta -gluco
se containing 10% 1,3-linkages, The sulfate was located on C-6 of 64% of th
e 4-linked glucose residues, A third alkali-soluble polysaccharide rich in
galactose was also detected, The distribution of the glucomannan and galact
ose containing polysaccharides was inversely related to the algal cell size
, Potential functions of these alkali-soluble polymers are discussed in the
context of cell wall polysaccharide assembly.