VERSATILE INSERTION PLASMIDS FOR TARGETED GENOME MANIPULATIONS IN BACTERIA - ISOLATION, DELETION, AND RESCUE OF THE PATHOGENICITY ISLAND LEE OF THE ESCHERICHIA-COLI O157-H7 GENOME

Citation
G. Posfai et al., VERSATILE INSERTION PLASMIDS FOR TARGETED GENOME MANIPULATIONS IN BACTERIA - ISOLATION, DELETION, AND RESCUE OF THE PATHOGENICITY ISLAND LEE OF THE ESCHERICHIA-COLI O157-H7 GENOME, Journal of bacteriology, 179(13), 1997, pp. 4426-4428
Citations number
14
Categorie Soggetti
Microbiology
Journal title
ISSN journal
00219193
Volume
179
Issue
13
Year of publication
1997
Pages
4426 - 4428
Database
ISI
SICI code
0021-9193(1997)179:13<4426:VIPFTG>2.0.ZU;2-E
Abstract
A system of versatile insertion plasmids was constructed that permits efficient delivery of the target sites of an ultra-rare-cutting endonu clease and the recombinase FLP into preselected sites of the bacterial genome. With the help of this system, the pathogenicity island LEE of the Escherichia coli 0157:H7 genome was as excised and isolated in vi tro, deleted in vivo, rescued as a plasmid, and transferred into anoth er strain.