CLONING AND CHARACTERIZATION OF THE STREPTOMYCES-PEUCETIUS DNMZUV GENES ENCODING 3 ENZYMES REQUIRED FOR BIOSYNTHESIS OF THE DAUNORUBICIN PRECURSOR THYMIDINE DIPHOSPHO-L-DAUNOSAMINE

Authors
OTTEN SL GALLO MA MADDURI K LIU XC HUTCHINSON CR
Citation
Sl. Otten et al., CLONING AND CHARACTERIZATION OF THE STREPTOMYCES-PEUCETIUS DNMZUV GENES ENCODING 3 ENZYMES REQUIRED FOR BIOSYNTHESIS OF THE DAUNORUBICIN PRECURSOR THYMIDINE DIPHOSPHO-L-DAUNOSAMINE, Journal of bacteriology, 179(13), 1997, pp. 4446-4450
Citations number
33
Categorie Soggetti
Microbiology
Journal title
Journal of bacteriology → ACNP
ISSN journal
00219193
Volume
179
Issue
13
Year of publication
1997
Pages
4446 - 4450
Database
ISI
SICI code
0021-9193(1997)179:13<4446:CACOTS>2.0.ZU;2-1
Abstract
Characterization of the dnmZ, dnmU, and dnmV genes from the daunorubic in-producer Streptomyces peucetius by DNA sequence analysis indicated that these genes encode a protein of unknown function plus a putative thymidine diphospho-1-keto-6-deoxyglucose-3(5)-epimerase and thymidine diphospho-1-ketodeoxyhexulose reductase, respectively. Inactivation o f each of the three genes by gene disruption and replacement in the wi ld-type strain demonstrated that all of them are required for daunosam ine biosynthesis.