Tritium/protium fractionation near and inside DNA

Citation
F. Baumgartner et Ma. Kim, Tritium/protium fractionation near and inside DNA, J RAD NUCL, 243(2), 2000, pp. 295-298
Citations number
13
Categorie Soggetti
Inorganic & Nuclear Chemistry
Journal title
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
ISSN journal
02365731 → ACNP
Volume
243
Issue
2
Year of publication
2000
Pages
295 - 298
Database
ISI
SICI code
0236-5731(200002)243:2<295:TFNAID>2.0.ZU;2-W
Abstract
The fractionation factor of tritium between water and DNA as well as betwee n water and the first hydration shell of DNA is determined. For this purpos e the sublimation kinetics of water from DNA dissolved in water were determ ined at -200 degreesC and tritium was measured in the remaining water free DNA. The last sublimating water fractions showed a tritium level of about 1 .4, the residual water free DNA about 1.9 units above the bulk, water. The tritium accumulation inside and near DNA is attributed to the thermodynamic triton-proton exchange isotope effect existing between the strong hydrogen bridges of water and weaker hydrogen bridges as well as inside DNA as betw een the first hydration shell and DNA.