Enzymatic acidolysis of tristearin with lauric and oleic acids to produce coating lipids

Triacylglycerols with potential for coating application were prepared by ac idolysis of tristearin with lauric and oleic acids using Lipozyme 1M60 lipa se in n-hexane. The effects of reaction parameters such as time, temperatur e, substrate mole ratio, water content, enzyme load, and enzyme reuse were studied. Five-gram scale synthesis was carried out to obtain the melting pr ofile of products by differential scanning calorimetry (DSC). An acceptable melting profile was obtained for the product obtained with a 1:4:1 (triste arin/lauric acid/oleic acid) mole ratio of reactants. The DSC melting peak for this product was 31.4 degreesC. Synthesis of 1200 g of this product was carried out at a 1:4:1 substrate ratio in a stirred tank batch reactor und er optimal conditions. The reaction product, purified by short-path distill ation, was coated onto crackers and studied for its moisture inhibition abi lity, under water vapor-saturated atmosphere, in a desiccator over differen t time intervals. The effectiveness of the synthesized lipid as a coating m aterial was compared against uncoated crackers as a control and with cocoa butter-coated crackers. The synthesized lipid was better in preventing mois ture absorption than cocoa butter.