Analysis of mutations in the p53 tumor suppressor gene and Ki- and Ha-ras proto-oncogenes in hepatic tumors of European flounder (Platichthys flesus)

DNA from five tumors, three other-injured livers and two normal liver tissu e samples from the European flounder were analyzed for mutations in exons 5 -8 of the tumor suppressor gene p53 and at codons 12, 13 and 61 of Ha- and Ki-l as proto-oncogenes. No tumor-specific mutations were identified by dir ect sequencing and single-strand conformation polymorphism of these genes. A number of silent polymorphisms were noted in p53. In addition to a need f or more extensive analyses of flounder liver tumor samples for ras or p53 m utations, other cancer-related genes should be investigated. (C) 2000 Elsev ier Science Ltd. All rights reserved.