Attenuation of hypertension by systemic delivery of retroviral vector containing type I angiotensin II receptor antisense cDNA

Citation
Py. Reaves et al., Attenuation of hypertension by systemic delivery of retroviral vector containing type I angiotensin II receptor antisense cDNA, METHODS, 22(3), 2000, pp. 211-218
Citations number
23
Categorie Soggetti
Biochemistry & Biophysics
Journal title
METHODS-A COMPANION TO METHODS IN ENZYMOLOGY
ISSN journal
10462023 → ACNP
Volume
22
Issue
3
Year of publication
2000
Pages
211 - 218
Database
ISI
SICI code
1046-2023(200011)22:3<211:AOHBSD>2.0.ZU;2-R
Abstract
Despite recent strides in the traditional pharmacological therapies in the control and management of hypertension, a successful prevention and cure fo r this disease by conventional drug strategy remain at a standstill. We hav e begun to investigate the conceptual possibility of the use of gene therap y in the control of hypertension. In this article we describe an experiment al protocol that provides proof of the principle that antisense (AS) inhibi tion of Type I angiotensin II receptor (AT(1)-R) could prevent development of hypertension on a longterm basis. A retrovirus-based vector has been use d to deliver AT(1)R-AS with high efficiency that attenuates development of high blood pressure and hypertension-associated cardiac and Vascular pathop hysiology in the spontaneously hypertensive rat. (C) 2000 Academic Press.