Interferon beta-1b increases interleukin-10 in a model of T cell-microgliainteraction - Relevance to MS

Background: The modes of action of interferon beta (IFN-beta) in MS remain unclear, but enhanced levels of the anti-inflammatory cytokine interleukin- 10 (IL-10) in the CSF of patients with MS may be a marker of its prognostic efficacy. Objective: To examine potential mechanisms by which IL-10 may be increased by IFN-beta in the milieu of the CNS, Methods: A model of T cell interaction with microglia in vitro was used. Production of cytokines was monitored by measuring the levels of various cytokine proteins, using ELISA . Results: Pretreatment of T cells with IFN-beta potentiates the production of IL-10 when they interact with adult human microglia, human fetal microg lia, or U937 cells treated with phorbol-12-myristate-13-acetate (PMA) and I FN-gamma. The enhancing effect of IFN-beta on IL-10 requires cell-cell cont act, but does not seem to depend on pathways implicated in microglia-T cell interactions, involving CD40, CD23, and B7. In contrast to IL-10, IFN-beta inhibits the production of other cytokines, including tumor necrosis facto r-alpha (TNF-alpha), IL-1 beta, IL-4, IL-12, and IL-13. Conclusions: The in crease of IL-10 in microglia-T cell interaction by IFN-beta together with a decrease of other cytokines may lead to a noninflammatory milieu in the CN S. This mechanism could contribute to the efficacy of IFN-beta in MS.