A pathology-MRI study of the short-T2 component in formalin-fixed multiplesclerosis brain

Objective: To determine the pathologic basis of areas not exhibiting signal of the short-T2 component of the T2 relaxation distribution in MS, as stud ied in formalin-fixed brain. Background: A myelin-specific MRI signal would be of great importance in assessing demyelination in patients with MS. Evi dence indicates that the short-T2 (10 to 50 millisecond) component of the T 2 relaxation distribution originates from water in myelin sheaths. The auth ors present two cases of MS in which the anatomic distribution of the short -T2 component was correlated with the pathologic findings in postmortem for malin-fixed brain. Method: One half of the formalin-fixed brain was suspend ed in a gelatin-albumin mixture cross-linked with glutaraldehyde, and scann ed with a 32-echo MRI sequence. The brain was then cut along the center of the 5-mm slices scanned, photographed, dehydrated, and embedded in paraffin . Paraffin sections, stained with Luxol fast blue and immunocytochemically for 2',3'-cyclic nucleotide 3'-phosphohydrolase for myelin and by the Biels chowsky technique for axone, were compared with the distribution of the amp litude of the short-T2 component of the comparable image slices. Results: T he anatomic distribution of the short-T2 component signal corresponded to t he myelin distribution. Chronic, silent MS plaques with myelin loss correla ted with areas of absence of short-T2 signal. The numbers of axone within l esions were reduced, but many surviving axons were also seen in these areas of complete loss of myelin. Conclusion: In formalin-fixed MS brains the sh ort-Ta component of the T2 relaxation distribution corresponds to the anato mic distribution of myelin. Chronic, silent demyelinated MS plaques show ab sence of the short-Ta component signal. These results support the hypothesi s that the short-T2 component originates from water related to myelin.