Ev. Gogol et al., Amperometric biosensors based on nafion coated screen-printed electrodes for the determination of cholinesterase inhibitors, TALANTA, 53(2), 2000, pp. 379-389
Screen-printed electrodes coated with the nafion layer have been investigat
ed for cholinesterase biosensor design. The butyrylcholinesterase (ChE) Fro
m horse serum was immobilised onto the nafion layer by cross-linking with g
lutaraldehyde vapours. The biosensors obtained showed better long-term stab
ility and lower wet-king potential in comparison to those obtained with no
nafion coating. The sensitivity of a biosensor toward organophosphate pesti
cides is not affected by the nafion coating. The detection limits were foun
d to be 3.5 x 10(-7) M for trichlorfon and 1.5 x 10(-7) M for coumaphos. (C
) 2000 Elsevier Science B.V. All rights reserved.