Amperometric biosensors based on nafion coated screen-printed electrodes for the determination of cholinesterase inhibitors

Citation
Ev. Gogol et al., Amperometric biosensors based on nafion coated screen-printed electrodes for the determination of cholinesterase inhibitors, TALANTA, 53(2), 2000, pp. 379-389
Citations number
46
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
TALANTA
ISSN journal
00399140 → ACNP
Volume
53
Issue
2
Year of publication
2000
Pages
379 - 389
Database
ISI
SICI code
0039-9140(20001101)53:2<379:ABBONC>2.0.ZU;2-7
Abstract
Screen-printed electrodes coated with the nafion layer have been investigat ed for cholinesterase biosensor design. The butyrylcholinesterase (ChE) Fro m horse serum was immobilised onto the nafion layer by cross-linking with g lutaraldehyde vapours. The biosensors obtained showed better long-term stab ility and lower wet-king potential in comparison to those obtained with no nafion coating. The sensitivity of a biosensor toward organophosphate pesti cides is not affected by the nafion coating. The detection limits were foun d to be 3.5 x 10(-7) M for trichlorfon and 1.5 x 10(-7) M for coumaphos. (C ) 2000 Elsevier Science B.V. All rights reserved.