Induction of antitumor activity of T-lymphocytes by antigen-presenting cells produced from blast cells of patients with acute leukemia

Aim. To produce antigen-presenting cells (APC) from tumor cells of patients ,vith acute leukemia and To induce autologous cytotoxic firmer-specific T-l ymphocytes in vivo and in vitro. Material and methods, Culture examinations, flow cytofluorometry were made in 3 patients with acute myeloid leukemia (AML) and 1 patient with acute ly mphoblastic leukemia (ALL). Results, Culturing of blast cells in the presence of growth factors produce d APC in 4 patients with acute leukemia. The use of fibronectin layer with added collagen proved most effective for the above production. In culture, APC induced autologous tumor-specific T-lymphocytes which selectively lysed tumor cells. Pilot clinical trial was made of dendritic cells suspension i n a patient with resistant AML. Conclusion, A new perspective immunotherapy of acute leukemia is proposed.