Comparison of chronic lymphocytic leukemia forms with different mutationalstatus of immunoglobulin variable region genes

Aim. To compare forms of chronic lymphoid leukemia (CLL) regarding mutation al status of immunoglobulin variable genes. Material and methods. We have compared clinical, prognostic and immunopheno typic data obtained on 25 cases with different mutational status of IgV gen es. There were 10 patients in the mutated group (median age 49.2 years, mal e to female ratio=7:3), and 15 patients in unmutated group (median age 46.5 , M:F=13:2). Results, Statistically significant differences were noted in overall surviv al and CD38 expression. 5-year overall survival in unmutated group was 35%, in mutated group 80% (p=0.07). In unmutated group CD38 was expressed on mo re than 50% of cells in 7 out of 14 patients, while in the mutated group in 0 of 8 patients (p=0.007). We noted high frequency of VH 1-69 gene usage i n unmutated group (7 of 15 patients), while in mutated group it was used in only 1 case of 10. Conclusion. We confirm the differences between groups of CLL with different mutational status of IgV genes. Highly restricted usage of VH-genes and CD 38 expression possibly suggest that unmutated group also arises from antige n driven cells.