High level production, characterization and construct optimization of the ionotropic glutamate receptor ligand binding core

Authors
Chen, GQ Gouaux, E
Citation
Gq. Chen et E. Gouaux, High level production, characterization and construct optimization of the ionotropic glutamate receptor ligand binding core, TETRAHEDRON, 56(48), 2000, pp. 9409-9419
Citations number
40
Categorie Soggetti
Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
TETRAHEDRON
ISSN journal
00404020 → ACNP
Volume
56
Issue
48
Year of publication
2000
Pages
9409 - 9419
Database
ISI
SICI code
0040-4020(20001124)56:48<9409:HLPCAC>2.0.ZU;2-4
Abstract
Multimilligram quantities of active and pure GluR2-S1S2, the recombinant li gand binding core of the AMPA-sensitive GluR2 receptor, were produced by pr eparative folding of the solubilized inclusion bodies expressed in 11 of Es cherichia coli cell culture. The biochemical properties and biological acti vities of folded protein were characterized and the protein construct was o ptimized for three-dimensional structural studies. (C) 2000 Elsevier Scienc e Ltd. All rights reserved.