Pmb. Fernandes et al., EFFECT OF HYDROSTATIC-PRESSURE ON A MUTANT OF SACCHAROMYCES-CEREVISIAE DELETED IN THE TREHALOSE-6-PHOSPHATE SYNTHASE GENE, FEMS microbiology letters, 152(1), 1997, pp. 17-21
Mutants Saccharomyces cerevisiae deleted on the trehalose-6-phosphate
synthase gene (tps1) and their parental wild-type cells were submitted
to hydrostatic pressure in the range of 0-200 MPa. Experimental evide
nce showed that viability for both strains decreased with increasing p
ressure and that tps1 mutants, unable to accumulate trehalose, were mo
re sensitive to hydrostatic pressure than the wild-type cells. Additio
nally, both tps1 and wild-type cells in the stationary phase, when the
re is an accumulation of endogenous trehalose, were more resistant to
pressure than proliferating cells. Under these conditions, mutant cell
s were also more sensitive to pressure treatment than the wild type. T
he present work also showed that mild pressure pretreatment did not in
duce hydrostatic pressure resistance (barotolerance) in yeast cells.