The configurations of the four geometric mer - isomers in a new [Co(3,3 -tr
i)(amp)Cl](2+) [3,3 - tri = N - (3-aminoethyl)-1,3-propanediamine; amp = 2
- (aminomethyl) pyridine] system were assigned by using 2D NMR techniques s
uch as gCOSY and NOESY. Solvent used was Me2SO - d(6) with the central peak
of the CD3 septet as the reference (C-13,delta = 39.37; H-1, delta = 2.49
relative to SiMe4). Comparing to the eluates from the chromatographic colum
n, cation exchange media used was Dowex 50W x 2 (H+ form, 200 similar to 40
0 mesh; Biorad Compauy), the first band contains the m2, the second band co
ntains the m4, the third band contains the mi and the fourth band contains
the m3.