Study on 2D NMR spectra of the four meridional isomers in [Co(3,3-tri)(amp)Cl](2+) system

The configurations of the four geometric mer - isomers in a new [Co(3,3 -tr i)(amp)Cl](2+) [3,3 - tri = N - (3-aminoethyl)-1,3-propanediamine; amp = 2 - (aminomethyl) pyridine] system were assigned by using 2D NMR techniques s uch as gCOSY and NOESY. Solvent used was Me2SO - d(6) with the central peak of the CD3 septet as the reference (C-13,delta = 39.37; H-1, delta = 2.49 relative to SiMe4). Comparing to the eluates from the chromatographic colum n, cation exchange media used was Dowex 50W x 2 (H+ form, 200 similar to 40 0 mesh; Biorad Compauy), the first band contains the m2, the second band co ntains the m4, the third band contains the mi and the fourth band contains the m3.