KV1.1 K+ channels identification in human breast carcinoma cells: Involvement in cell proliferation

Electrophysiological, immunocytochemical, and RT-PCR methods were used to i dentify a K+ conductance not yet described in MCF-7 human breast cancer cel ls. A voltage-dependent and TEA-sensitive K+ current was the most commonly observed in these cells. The noninactivating K+ current (I-K) was insensiti ve to iberiotoxin (100 nM) and charybdotoxin (100 nM) but reduced by alpha -dendrotoxin (alpha -DTX). Perfusion of (alpha -DTX reduced a fraction of I -K amplitude in a dose-dependent manner (IC50 = 0.6 +/- 0.3 nM). This DTX s ensitive I-K exhibited a voltage threshold at -20 mV and was not inactivate d. The time constant of activation was 5.3 a 2.2 ms measured at +60 mV. The averaged half-activation potential and slope factor values were 14 +/- 1.6 mV and 10 +/- 1.4, respectively. Immunocytochemical analysis demonstrated that plasma membrane was labeled by anti-Kv1.1 but not by anti-Kv1.2 nor an ti-Kv1.3 antibodies. Furthermore, only Kv1.1 mRNA was detected in MCF-7 cel ls. Incubation in 1 and 10 nlM alpha -DTX reduced cell proliferation by 20 and 30%, respectively. These data provide the first evidence of Kv1.1 K+ ch annels expression in MCF-7 cells and indicate that these channels are impli cated in cell proliferation. (C) 2000 Academic Press.