Genetically synthesized antibody-binding protein self-assembled on hydrophobic matrix

A unique antibody-binding protein, (E12B2)n, was genetically synthesized, w hich was characterized by a hydrophobic peptide, E12, at one terminus and a n antibody-binding peptide, B2, at the other. It was clarified by atomic fo rce microscopy (AFM) imaging that this protein was efficiently self-assembl ed on a hydrophobic solid surface. (E12B2)n self-assembled on a microplate exhibited an excellent performance of antibody-binding affinity. The propos ed design of antibody-binding protein seems promising in immobilizing antib ody molecules on hydrophobic solid surfaces.