Dimethyl suberimidate cross-linking of oligo(dT) to DNA-binding proteins

Dimethyl suberimidate is a bifunctional reagent that is used for cross-link ing the protein components of oligomeric macromolecules. In this report, di methyl suberimidate is shown to specifically crosslink oligo(dT) of varying lengths to the DNA-binding subunits of a multimeric helicase-primase encod ed by herpes simplex virus type 1. This result indicates that dimethyl sube rimidate and other imidoester cross-linking reagents may be useful for char acterizing the interaction of oligo(dT) with proteins that bind single-stra nded DNA.