Biofilm thickness variability investigated with a laser triangulation sensor

Measurement of the surface roughness and thickness of biological films is l aborious and usually destructive, thus hampering research in this area. We developed a laser triangulation sensor (LTS) set-up for the fast and nondes tructive measurement of these biofilm parameters during growth. Using LTS m easurements, the morphological development of a dichloromethane-(DCM) degra ding biofilm cultured on a wetted-wall column was studied. The measurements show that the biofilm develops faster at the entrance of the reactor. The biofilm consisted of a base film in which microbial colonies were embedded. The biofilm-surface area gradually increased by 23% compared to the bare s urface due to the formation of a large number of these colonies. The number and shape of these colonies were followed in time. Using LTS measurements, biofilms distinctly different in surface roughness could be distinguished in a laboratory trickling filter removing DCM from a waste gas. The consequ ences of the observed surface characteristics for the reaction-diffusion pr ocess in the biofilm and for the falling film hydrodynamics are discussed. (C) 2000 John Wiley & Sons.