Citation
I. Theodossiou et al., New expanded bed adsorbents for the recovery of DNA, BIOTECH LET, 22(24), 2000, pp. 1929-1933
Citations number
15
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492
→ ACNP
Volume
22
Issue
24
Year of publication
2000
Pages
1929 - 1933
Database
ISI
SICI code
0141-5492(200012)22:24<1929:NEBAFT>2.0.ZU;2-Z
Abstract
A 20-40 mum pellicular high density (similar to3.7 g cm(-3)) expanded bed m
aterial has been designed for the capture of DNA and other large macromolec
ules. Anion exchangers fashioned out of these supports exhibited dramatical
ly enhanced DNA binding capacities over commercial anion exchange adsorbent
s (6 mg ml(-1), c.f. similar to 50 mug ml(-1) at 10% breakthrough), due to
a combination of small particle and 'fuzzy' surface architecture created th
rough the coupling of polyethylene imine chains.