CALM-AF10 fusion gene in leukemias: simple and inversion-associated translocation (10;11)

A translocation (10;11)(p12;q14) was observed in two children, one with acu te eosinophilic leukemia and the other with acute T-cell lymphoblastic leuk emia. The presence of CALM-AFIO fusion was ascertained by reverse transcrip tase-polymerase chain reaction (RT-PCR) analysis. Fluorescence in situ hybr idization (FISH) analysis showed that AF10 gene splitting was associated wi th partial inversion of chromosome 11 in the first patient. In addition, FI SH analysis also determined the orientation of the CALM gene, 5' telomere t o 3' centromere on 11q. (C) 2000 Elsevier Science Inc. All rights reserved.