RB18A, whose gene is localized on chromosome 17q12-q21.1, regulates in vivo p53 transactivating activity

Among the different cellular factors that regulated p53 functions, we previ ously identified (P. Drane st at, Oncogene, 15: 3013-3024, 1997) RB18A, a n ew gene whose encoded M-r 205,000 protein interacted in vitro, through its COOH-terminal domain, with p53. Therefore, we analyzed the in vivo role of RB18A by measuring its effect on the transactivating activity of p53 on phy siological promoters. We herein demonstrated that RB18A, which interacted a lso in vivo with p53, activated Bax promoter and inhibited p21(Waf1) or IGF -BP3 promoters. In addition, fluorescence in situ hybridization mapping led to localizing the RB18A gene on chromosome 17q12-q21.1, loci associated wi th human cancers. This is the first demonstration that in vivo RB18A, in a protein-protein interaction, regulates p53 transactivating activity.