Analysis of glutamate in beverages and foodstuffs by capillary electrophoresis with laser-induced fluorescence detection

A simple, sensitive, and robust method has been developed for the separatio n and quantification of glutamate in beverages and foodstuffs. Samples were derivatized with 5 x 10(-4) M fluorescein isothiocyanate. Glutamate was se parated by capillary electrophoresis and detected by laser-induced fluoresc ence wi ih 488-nm radiation from an argon ion laser Response was linearly d ependent on concentration between 10(-7) and 10-4 M. The limit of mass dete ction was 16 amor and the limit of concentration detection was 5.4 x 10(-10 ) M. Intra- and interday precision were 3.3% RSD (n = 11) and 4.8% RSD (n = 7), respectively. The method has been applied to beverages and other foods .