D. Franco et al., Multiple transcriptional domains, with distinct left and right components,in the atrial chambers of the developing heart, CIRCUL RES, 87(11), 2000, pp. 984-991
During heart development, 2 fast-conducting regions of working myocardium b
alloon out from the slow-conducting primary myocardium of the tubular heart
. Three regions of primary myocardium persist: the outflow tract, atriovent
ricular canal, and inflow tract, which are contiguous throughout the inner
curvature of the heart. The contribution of the inflow tract to the definit
ive atrial chambers has remained enigmatic largely because of the lack of m
olecular markers that permit unambiguous identification of this myocardial
domain. We now report that the genes encoding atrial natriuretic factor, my
osin light chain (MLC) 3F, MLC2V, and Pitx-2, and transgenic mouse lines ex
pressing nlacZ under the control of regulatory sequences of the mouse MLC1F
/3F gene, display regionalized patterns of expression in the atrial compone
nt of the developing mouse heart. These data distinguish 4 broad transcript
ional domains in the atrial myocardium: (1) the atrioventricular canal that
will form the smooth-walled lower atrial rim proximal to the ventricles; (
2) the atrial appendages; (3) the caval vein myocardium (systemic inlet); a
nd (4) the mediastinal myocardium (pulmonary inlet), including the atrial s
epta. The pattern of expression of Pitx-2 reveals that each of these transc
riptional domains has a distinct left and right component. This study revea
ls for the first time differential gene expression in the systemic and pulm
onary inlets, which is not shared by the contiguous atrial appendages and p
rovides evidence for multiple molecular compartments within the atrial cham
bers. Furthermore, this work will allow the contribution of each of these m
yocardial components to be studied in congenitally malformed hearts, such a
s those with abnormal venous return.