An oxygen-insensitive reagentless glucose biosensor based on osmium-complex modified polypyrrole

An optimized material for the development of reagentless oxygen-independent biosensors based on conducting polymers is described. Considering the prer equisites for a fast electron transfer between a redox enzyme and the elect rode surface via an electron-hopping mechanism, an Os-complex-modified pyrr ole derivative with a long, flexible spacer chain has been synthesized. Cop olymerization of the new mediator-modified pyrrole monomer with pyrrole was optimized aiming on a higher mediator loading in the film. The feasibility of this material for the development of reagentless oxygen-independent bio sensors is demonstrated by entrapment of a PQQ-dependent glucose dehydrogen ase isolated from Erwinia sp. 34-1 within this electrochemically grown redo x-polymer network.