Mercury film electrode as a sensor for the detection of DNA damage

Authors
Kubicarova, T Fojta, M Vidic, J Havran, L Palecek, E
Citation
T. Kubicarova et al., Mercury film electrode as a sensor for the detection of DNA damage, ELECTROANAL, 12(17), 2000, pp. 1422-1425
Citations number
12
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ELECTROANALYSIS
ISSN journal
10400397 → ACNP
Volume
12
Issue
17
Year of publication
2000
Pages
1422 - 1425
Database
ISI
SICI code
1040-0397(200011)12:17<1422:MFEAAS>2.0.ZU;2-R
Abstract
Cleavage of supercoiled (sc) plasmid DNA by restriction endonucleases and b y deoxyribonuclease I was detected by means of AC voltammetry at mercury fi lm on glassy carbon electrode (MFE). Using this technique, it was possible to detect both single- and double-strand breaks in DNA. MFE modified with s cDNA was used as a biosensor for the detection of DNA-cleaving species.