Determination of enantiomeric purity of hyoscyamine from scopolia extract using HPLC-CD system without chiral separation

Authors
Kudo, K Iwaya, K Yomota, C Morris, S Saito, M
Citation
K. Kudo et al., Determination of enantiomeric purity of hyoscyamine from scopolia extract using HPLC-CD system without chiral separation, ENANTIOMER, 5(3-4), 2000, pp. 369-375
Citations number
19
Categorie Soggetti
Chemistry
Journal title
ENANTIOMER
ISSN journal
10242430 → ACNP
Volume
5
Issue
3-4
Year of publication
2000
Pages
369 - 375
Database
ISI
SICI code
1024-2430(2000)5:3-4<369:DOEPOH>2.0.ZU;2-2
Abstract
Enantiomeric ratio of hyoscyamine from Scopolia extract was determined by c hiral HPLC-CD analysis. It was found that circular dichroism (CD) detection allowed the analysis of the sample without any special pretreatment wherea s UV detection required an ammonia-ether extraction. To obtain a shorter an alysis time for the determination, reversed-phase HPLC-CD analysis was appl ied by using a g-factor calibration curve (EE% vs. CD/UV). The analysis tim e was shortened from 35 to 18 min. EE% values obtained were consistent with those by chiral HPLC analysis.