Detection of a b3a3 type chimera in chronic myeloid leukemia by double hybridisation of RT-PCR products

The standard RT-PCR method performed on RNA of a chronic myeloid leukemia p atient resulted in a product of unusual size. Hybridisation to a]probe cont aining the a2 sequences yielded a very faint band. Rehybridisation of the s ame blot to b3 sequences has given a film signal. Based on the size of the product and on results of hybridisation tests, a translocation, resulting i n a b3a3 juxtaposition, was supposed. The direct sequencing of the KT-PCR p roduct has proven this hypothesis. We concluded, that double hybridisation of RT-PCR products of standard methods is a useful tool in detection of rar e bcr-abl chimeras.